Human health is strongly intertwined with the health of the microbiome. A Large Number of Studies Have Shown that the Human Microbiome Plays a Critical Role in Tumoriganes, Tumor Progression, and the Efficiency of ANTICANCER Therapies Mechanisms.

The microbiome can be used as a tumor marker and is expected to be a novel therapeutic target. However, as part of the tumor immune microenvironment, the intratumoral microbiome of lung cancer, which is the leading tumor in terms of new cases and deaths world, is what to more digested and the lifes Activities of Various Cell Types in Lung Cancer Tumor Tissues Compared to the Microbiota of other parts of the human body.

Previous studies have revised the molecular mechanism by which the immunosuppressive microenviron induced by intratumoral fungi promotes the development of lung cancer and confeated that the enrichment of Aspergillus Sydowii in Tumors is positively correlated with adverse outcomes of lung cancer patients.

However, the molecular mechanism of how the Intratumoral Microbiome Interacts with Single-Cell Hosts during the Development of Lung Cancer Remains Unclear.

A Team of Researchers from China Integrated Six Published Published Single-Cell RNA Sequencing Datasets with 149 Lung Cancer PATINT TISSUE SAMPLES-SINCLUDING LUNCLUDING LINDACER, NORMAL TISSUES Adj The cancer and brain metastases –nd integrated the saahmi tool for mining microbial sequence information and single-call analysis by removing potential microbial contamination and False-positive sequences and FATCH EFFECT DURING MULTI-DATASETS Integration.

They obtained the True Microbial Sequences Associated with Individual Host Cells and Investigated The Relationship Between Different Host Host Cells and Specific Microbiota with Tissue Tissue Typsue Tissue Typs The Single-Cell Resolution. Their study is Published in the journey hlife,

Their findings sugges that host cell composition varies during Lung Cancer Progression and Metastasis. The proportions and numbers of bacterial- and fungal-host cells Fungal-hassociated cells in all lung cancer and its Paratumral and Brain Metastatic Tissue Samples.

The proportion of fungal -ssociated cells was the highest in the primary lung cancer lesions compared to brain metastases and paratumral normal tissues, beereas the proportion of Bacterial-buses Wasociated Cells Abundant in Brain Metastatic Tissues Compared to Primary Lung Tumor and Paratumoral Tissues. Meanwhile, there was differences in the proportions of cell types in samples of different tissue types, and there were significant directions in the distribution of fungi and bacteria.

The expression of host cell type-specific genes was associated with the presence of specific bacteria, and the presence of specific bacteria significantly and defense Expression in their co-locklized or residence host cells by modulating the activity of a variety of pathways related to RRNA processing, cellular responses to stimuli and stress, RNA and Protein metabolism, Etc.

There was an effect of the presence or absence of Bacteria on Host Cell Communication and Gene Expression in Different T Cell Subtypes. There was differential regulation of host macrophage by gene expression such as Plp1 by Bacteria and Fungi. It was upregulated in macrophages exposed to bacteria, but downregulated in fungus-hassociated macrophages.

In addition, the study found that some microorganisms were associated with sample type and the smoking status of Lung Cancer Patients. In terms of smoking frequency, various fungi such as pichia, saccharomyces, pyricularia, and sugiyamaella showed differential abundance in Normal, Primary Lung Cancer, Primary Lung Cancer Tissues.

In summary, revealing the complex interaction between the host and microbiome at the single-cell level may be a new breakthrough to explore the factor the factoring the factories, developastasis and future of Lung Cancer, and May Provide New Clues for the Realization of Personalized Cancer Treatment in Clinical Practice in the future.

Provided by tsinghua university press