Using A New Technology and Computational Method, Researchers from Fred Hutch Cancer Center and The University of Texas MD Anderson Cancer Center have uncovered a biomarker capable of cacurate Rain tumors and breast cancers.

In the study, Published Today in ScienceThe Researchers Discovered that the Amount of a Specific Enzyme, RNA Polymerase II (RNAPII), Found on Histone Ganes was Associated With Tumor Aggressivance and recurrent

Hyper-Elvated Levels of RNAPII on these histone genes indicate cancer over-proliferation and potentially contribute to chromosomal changes. These Findings point to the use of a new genomic technology as a potential cancer diagnostic and program tool, whichoundwalic improvement Oncology Approaches.

“It has been overlooked that Histone Ganes BE fessor of bioinformatics and computational biology at md anderson.

“This is beCAuse Current RNA sequencing methods ew experience Samples from Multiple Cancer Types to Enhance Tumor Diagnosis and Prognosis. “

New Technology Productions Better Quality Data from Samples Stored Over Decades

The Results of the Study Were Made Possible by a New Profiling Technology Developed in the Lab of Steven Henikoff, Ph.D., Co-FIRST AAUTHOR AND DEAR and Professor in the Basic Sciences Division ERS to Better Study Gene Expression Using Formalin-Fixed, Paraffin-Embeded (FFPE) Samples.

Tissue biopsies a.

The New Technology-Clevage Under Targeted Accessible Chromatin (Cutac)-Focuses on Small, Fragmented DNA Non-Coding Sequenses Where Rnapi Bind, Located on the Same Chromosome Lowing scientists to directly measure gene transcription activity from the DNA.

WHEN Examining Clinical Samples Using Cutac Technology Across Various Cancer Types, The Researchers Found That The Expression of Histone Genes was Consistent and Significant Mal tissue samples.

Histone Proteins Provide Essential Structural Support for DNA in Chromosomes, Acting as Spools Around Whoch DNA Strands Wrap. These proteins have been well studed, but most current tools to study gene expression relay on rna sequencing. Histone RNA is unique in that its structure prevents the RNA Molecules from Being Detected by Current Methods.

Thus, The Expression of Histone Genes May Be Significantly undress in Tumor Samples. The Researchers Hypotesized that the Increased Provaliation of Cancer Cells Leads to a very elevated Expression, or Hypertranscription, of Histones to Meet the Added Demands of Cell Replication and Division.

RNAPII EXPRESSION Correlates with and Predicts Cancer AGGRESSIVENESS

To test their hypothesis, The Researchers used cutac profileing to examine and map rnapii, which transferbes dna into precursors of messenger rna. He6 FFPE Samples from Patients with Meningioma – A Common and Benign Brain Tumor – A novel computational approach to integrate this data with nearly 1,300 publishes Responding clinical outcomes.

In tumor samples, the rnapii enzyme signals found on history genes WRELYLY ALELY ALELY ALELY ALELY to distinguish betteren cancer and normal samples.

RNAPII Signals on Histone Genes also correlated with Clinical Grades in Meningiomas, Accurately predicting rapid recurnce as well as the tendency for whole-acquires. Using this Technology on Breast Tumor FFPE SAMPLES from 13 Patients with Invasive Breast Cancer also Predicted Cancer AGGRESSVENESS.

“The Technique We Developed to Examine preserved tumor samples a previous overlooked mechanism of cancer aggressiveness,” Said henikoff, who is also also a also ALSO ALSO ALSO ALSO ALSO . “Identifying this Mechanism sugges it could be a new test to diagnose cancers and possible treatment them.”

Zheng and Colleagues Plan to use this technology on